Translational_Unit

Part:BBa_K2207019:Design

Designed by: Shisheng Li   Group: iGEM17_ZJU-China   (2017-10-21)


TRPV1-antiEGFP-2A-EGFP-Ferritin light chain-flag-Ferritin heavy chain


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 787
    Illegal PstI site found at 1183
    Illegal PstI site found at 1202
    Illegal PstI site found at 1870
    Illegal PstI site found at 2476
    Illegal PstI site found at 2557
    Illegal PstI site found at 4519
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 394
    Illegal NheI site found at 1402
    Illegal NheI site found at 2956
    Illegal PstI site found at 787
    Illegal PstI site found at 1183
    Illegal PstI site found at 1202
    Illegal PstI site found at 1870
    Illegal PstI site found at 2476
    Illegal PstI site found at 2557
    Illegal PstI site found at 4519
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1313
    Illegal BglII site found at 1814
    Illegal BglII site found at 2950
    Illegal BamHI site found at 2836
    Illegal XhoI site found at 2783
    Illegal XhoI site found at 3886
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 787
    Illegal PstI site found at 1183
    Illegal PstI site found at 1202
    Illegal PstI site found at 1870
    Illegal PstI site found at 2476
    Illegal PstI site found at 2557
    Illegal PstI site found at 4519
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 787
    Illegal PstI site found at 1183
    Illegal PstI site found at 1202
    Illegal PstI site found at 1870
    Illegal PstI site found at 2476
    Illegal PstI site found at 2557
    Illegal PstI site found at 4519
    Illegal AgeI site found at 2594
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 53
    Illegal BsaI site found at 4190
    Illegal BsaI.rc site found at 461
    Illegal BsaI.rc site found at 1594
    Illegal BsaI.rc site found at 2105
    Illegal BsaI.rc site found at 2431
    Illegal SapI.rc site found at 2508


Design Notes

This whole part is quite large and can be hard to clone it into vectors. We tried several times to construct the plasmid to express this part. It does take patience and efforts.To express it in Saccharomyces cerevisiae, we select aaaaaaatg as the kozac sequenc


Source

The original sequence are gotten from add gene(plasmid #79649). We used PCR to amplify these sequence.

References